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Matrix Science mascot software version 2 7 0
Mascot Software Version 2 7 0, supplied by Matrix Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mascot+software+version+2+2+0/mascot+software/pm41432296-142-18-22
Average 86 stars, based on 1 article reviews
mascot software version 2 7 0 - by Bioz Stars, 2026-09
86/100 stars

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Article Title: One-step purification of assembly-competent tubulin from diverse eukaryotic sources
Article Snippet: The resulting peptide mixtures were extracted with 5% formic acid/50% acetonitrile and analyzed by liquid chromatography–tandem mass spectrometry on an Ultimate nanoLC system (Thermo Scientific Dionex, Amsterdam, Netherlands) interfaced online to a LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) via a TriVersa robotic nanoflow ion source (Advion BioSciences, Ithaca NY) as described in Junqueira et al. (2008 ). .. Acquired spectra were searched against the National Center for Biotechnology Information protein database without species restriction using MASCOT software, version 2.2.0 (Matrix Science, London, United Kingdom), with the following settings: precursor mass tolerance was ±5 ppm; fragment mass tolerance was ±0.5 Da; variable modifications were propionamide (C), oxidation (M), and acetylation (protein N-terminus); enzyme was trypsin; two missed cleavages were allowed. .. Hits were evaluated using Scaffold software, version 2_04_00 (Proteome Software, Portland, OR), with the following settings: minimal number of matching peptides, two; peptide probability, 95%; protein probability, 99%; protein false discovery rate was calculated as 0.1%.



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